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Image Search Results
Journal: Cancer Science
Article Title: Stanniocalcin 2 overexpression in castration‐resistant prostate cancer and aggressive prostate cancer
doi: 10.1111/j.1349-7006.2009.01117.x
Figure Lengend Snippet: Stanniocalcin 2 (STC2) overexpression in castration‐resistant prostate cancer (CRPC) cells. (a) Semiquantitative reverse transcription–polymerase chain reaction (RT‐PCR) validated that STC2 was overexpressed in the microdissected CRPC cells, compared with castration‐naïve prostate cancer (CNPC) cells and normal prostatic epithelial (NP) cells, which were also microdissected. β‐actin (ACTB) was used to quantify the cDNA contents. (b) Real‐time quantitative RT‐PCR showed overexpression of STC2 transcript in CRPC cells (samples 2–8) compared with that of CNPC cells (samples 9–15) and NP cells (sample 1). ACTB was used to quantify each of the cDNA contents, and the relative quantity (y axis) was calculated so that the expression in NP cells was one. Real‐time quantitative RT‐PCR was duplicated for each sample (white and black columns). (c) Northern blot analysis showed a high level of STC2 expression in five prostate cancer cell lines (lanes 1–5), whereas its expression was hardly detectable in adult normal organs, including brain, heart, kidney, liver, lung, prostate, and testis (lanes 6–12). (d) Multiple tissue northern blot analysis indicated that STC2 was expressed only in normal pancreas, with no or very low expression in adult normal organs. The length of the STC2 transcript was approximately 5.4 kb. P.B.Leukocyte, peripheral blood leukocyte.
Article Snippet:
Techniques: Over Expression, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Northern Blot
Journal: Cancer Science
Article Title: Stanniocalcin 2 overexpression in castration‐resistant prostate cancer and aggressive prostate cancer
doi: 10.1111/j.1349-7006.2009.01117.x
Figure Lengend Snippet: Immunohistochemical analysis of stanniocalcin 2 (STC2) in prostate cancer tissues. Immunoreactivity with anti‐STC2 antibody was observed in castration‐resistant prostate cancers (CRPCs) and castration‐naïve prostate cancers (CNPCs) with high Gleason scores (GS), exhibiting strong positive immunostaining in the cytoplasm of prostate cancer cells. (a) The representative picture of CRPC (×200); (b) the representative picture of CNPC with GS 10 (×200); (c) the representative picture of CNPC with GS 7 (×200). Adjacent normal prostatic epithelium revealed very weak or no signal for STC2 ([b,c] arrowheads). (d) Correlation between STC2 immunohistochemical (IHC) score and GS in CNPCs. There were significant differences in STC2 IHC scores between the GS 2–6 group (n = 12) and the GS 8–10 group (n = 8), and between the GS 7 (n = 33) and GS 8–10 groups (Mann–Whitney U‐test with Bonferroni method; P = 0.0006 and P = 0.0004, respectively).
Article Snippet:
Techniques: Immunohistochemical staining, Immunostaining, MANN-WHITNEY